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Stereology determining sampling precision
Stereology determining sampling precision











The greater the differences in samples the better the proportionator works. The proportionator is able to significantly reduce the work involved in situations where the variance is high. This is the difference seen between samples. The proportionator improves counting by directly addressing the issue of variance. The new method is known as the proportionator. The method was improved over the years and has recently seen a jump in efficiency. This method employed 2 concepts: a method of counting objects of varying size, shape, orientation, and distribution seen on a surface along with a method that allowed counting in 3-dimensions. The first unbiased counting method was developed in 1984. The number of grains of a mineral or the number of cells in an organ are typical questions to answer. One of the more difficult microscopic questions is the number of particles in a solid. It is more useful when the objects are fixed in position such as grains of temper in a ceramic, trees in a forest, cells in an organ, or metals in an alloy. The importance of systematic sampling is not in a situation where the objects can be rearranged such as a group of students. No one student can predict if they will be picked or not although students can line up knowing that if they are picked, then their friends are also picked.Ĭlearly, using systematic sampling is not as random as independent random sampling. The teacher begins with the first chosen student and then walks down the line of students touching every other students' head. If it is heads then the first student is chosen. This is where the first sample is chosen at random and the rest of the samples are chosen systematically. The most common approach in stereological studies is the systematic sampling approach. The first step was deciding what is to be studied. Getting the sampling correct is the second big step in this process. A small piece of a pot may be removed and used to study the pot.Īs you might expect the manner in which the sample is chosen is important. A piece of tissue may be chosen or removed from a large organ to determine what is inside of the organ. For example, one or more rocks may be chosen as sampled of the rocks found in an ore deposit. A sample is a piece taken from the original object or group of objects and used to represent the bigger group. The work to complete tasks may be too arduous, too dangerous, too expensive, or destructive of the objects being studied. Can the entire deposit be evaluated without mining it in the first place? Is it possible to count the millions or billions of cells found in many tissues in an organism? Is it possible to determine the materials that make up a pot without destroying it? The solution is estimate the number of trees and get a good idea of how many trees there are.Īn important question might be, "How much copper can be retrieved by mining a given deposit?" Another important question might be, "If a drug is given to a patient, do any cells die?" Another interesting question to answer might be, "Even though these two ancient pots look alike, were they made from the same raw materials?"Įach of these questions is fairly difficult to directly measure. What does it matter if the answer is 23 million trees or 23 million and 5 trees? Besides, who is going to be able to count every single tree. It does not make sense to count every tree. Suppose that the question is, "How many trees are in a forest?" There might very well be millions of trees in any given forest. Estimation is a way to get an idea of how much there is without actually having to measure the value.Ī few examples should make this easier to understand. Other common geometric quantities are volume and the number of objects. A geometric quantity is something like length or surface area. Stereology is the science of estimating or measuring geometric quantities.













Stereology determining sampling precision